By Carolyn A. Staton, Claire Lewis, Roy Bicknell
Chapter 1 Endothelial mobile Biology (pages 1–38): Femke Hillen, Veerle Melotte, Judy R. van Beijnum and Arjan W. Griffioen
Chapter 2 Endothelial mobile Proliferation Assays (pages 39–50): Wen?Sen Lee
Chapter three Endothelial mobile Migration Assays (pages 51–64): Christos Polytarchou, Maria Hatziapostolou and Evangelia Papadimitriou
Chapter four Tubule Formation Assays (pages 65–87): Ewen J. Smith and Carolyn A. Staton
Chapter five Modelling the consequences of the Haemodynamic setting on Endothelial mobile Responses appropriate to Angiogenesis (pages 89–103): Gerard B. Nash and Stuart Egginton
Chapter 6 entire or Partial Vessel Outgrowth Assays (pages 105–121): Cindy H. Chau and William D. Figg
Chapter 7 Assaying Endothelial–Mural cellphone Interactions (pages 123–137): Melissa okay. Nix and Karen ok. Hirschi
Chapter eight Assays for Membrane and Intracellular Signalling occasions (pages 139–166): Vittorio Tomasi, Cristiana Griffoni, Spartaco Santi, Patrizia Lenaz, Rosa Anna Iorio, Antonio Strillacci and Enzo Spisni
Chapter nine Implantation of Sponges and Polymers (pages 167–182): Silvia P. Andrade, Monica A. N. D. Ferreira and Tai?Ping Fan
Chapter 10 Angiogenesis Assays within the Chick (pages 183–201): Andries Zijlstra, David Mikolon and Dwayne G. Stupack
Chapter eleven Corneal Angiogenesis Assay (pages 203–228): Siqing Shan and Mark W. Dewhirst
Chapter 12 Dorsal Air Sac version (pages 229–238): Sei Yonezawa, Tomohiro Asai and Naoto Oku
Chapter thirteen Chamber Assays (pages 239–263): Michael D. Menger, Matthias W. Laschke and Brigitte Vollmar
Chapter 14 Tumour versions: research of Angiogenesis in vivo (pages 265–291): Sven A. Lang and Oliver Stoeltzing
Chapter 15 Angiomouse: Imageable types of Angiogenesis (pages 293–310): Robert M. Hoffman
Chapter sixteen concepts and Advances in Vascular Imaging in Danio Rerio (pages 311–326): Kenna R. generators Shaw and Brant. M. Weinstein
Chapter 17 organic and medical Implications of Recruitment of Stem Cells into Angiogenesis (pages 327–340): Gianluigi Castoldi, Antonio Cuneo and Gian Matteo Rigolin
Chapter 18 tools for tracking of the Anti?Angiogenic job of brokers in sufferers: Novel Trial layout (pages 341–359): Shannon Smiley, Michael ok. ok. Wong and Shaker A. Mousa
Chapter 19 an summary of present Angiogenesis Assays: collection of Assay, Precautions in Interpretation, destiny requisites and instructions (pages 361–374): Robert Auerbach
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Extra info for Angiogenesis Assays: A Critical Appraisal of Current Techniques
2002). 14 CH1 ENDOTHELIAL CELL BIOLOGY VVOs span the entire thickness of vascular endothelium thereby providing a potential trans-endothelial connection between the vascular lumen and the extravascular space. They are large collections of vesicles and vacuoles focally expressed in the microvasculature that accompanies tumours, in venules associated with allergic inflammation and in the endothelia of normal venules, often in a parajunctional location. Structures of endothelial junctions vary along the vascular segments.
1994). , 2003). , 2003). Gene expression in in vitro models of angiogenesis Gene expression profiling has been applied to endothelial cells treated with different pro-angiogenic cytokines in various models to elucidate genes involved in angiogenesis. , 2001). , 2001). Variations in experimental set-ups will have contributed to the observed differences. An interesting observation comes from Gerritsen and colleagues (2003b), who studied the effects of HGF and VEGF on gene expression in endothelial cell culture.
Endothelial specific gene expression It has long been recognized that specific growth factors and cytokines act on endothelial cells by interaction with specific receptors on the endothelium. There are different vascular endothelial growth factor (VEGF) isoforms as well receptor isoforms (reviewed by Ferrara, 2004), that are expressed in endothelial cells. , 2001). Large-scale gene expression surveys have shed light on transcriptional differences between diverse types of cells. In addition, public data sets are available to mine expression data generated by various methods such as SAGE and microarray analysis.