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By W Roy Mason

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Extra resources for A practical guide to magnetic circular dichroism spectroscopy

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A transition hypotheses can then be advanced and tested within the framework of the molecule’s energy level scheme. For example, the sign and the approximate magnitude of the MCD terms expected can be predicted for a given transition and then compared with experiment. Thus, transitions that predict the wrong sign for the observed MCD term can be ruled out, and thereby narrow the possibilities. 2. PREDICTION OF SIGN AND APPROXIMATE MAGNITUDE OF MCD TERMS In order to predict MCD term sign and magnitude, the matrix elements in the appropriate term equations must be evaluated.

Again, trade-off compromise is necessary for good results. The best magnets are superconducting systems (fields up to 10 T), but these require liquid helium cryogenics for cooling the coils to below the critical temperatures of the windings. 3 shows a sketch of a typical single coil superconducting magnet. Current is introduced into the magnet windings by opening the superconducting (SC) switch. 3. Single-coil superconducting magnet. 44 MEASUREMENT OF MCD SPECTRA opening the switch. With the switch open, current from a suitable high-current power supply can then be impressed on the magnet coil.

4. OPTICAL ELEMENTS AND STRAY POLARIZATION EFFECTS 41 the spectral bandwidth that determines the spectral resolution, and acquisition data density. The signal-to-noise ratio in a measurement limits the ultimate sensitivity of the measurement and is often limited by the light flux that passes through the sample and reaches the PMT detector. The greater the flux, the better will be the signal-to-noise ratio. Light flux is limited by such experimental parameters as source brightness, the cross-sectional area of the light beam, and the various apertures, including the entrance and exit slits, which limit light passage through the spectrometer.

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